Anti-Human CD19-FITC/CD56-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail

This product is a FCM antibody cocktail made up of FITC Anti-Human CD19 [Clone: CB19] (Mouse IgG1, κ), PE Anti-Human CD56 Antibody [Clone: 5.1H11] (Mouse IgG1, κ), PE/Cyanine7 Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ) and PerCP Anti-Human CD45 Antibody [Clone: HI30] (Mouse IgG1, κ).; CD19 is a single-chain transmembrane glycoprotein expressed on B cells of all stages except plasma cells. It is a common marker for B cells. CD19 is also expressed in follicular dendritic cells. It forms complex with CD21 and CD84, which forms co-receptor with BCR. It takes part in B cell development, activantion and differentiation.; CD56 is also call neural cell adhesion molecule (NCAM), expressed on neurons, glia and skeletal muscle cells. In hematopoietic cells, CD56 is also expressed on NK cells and NKT cells. CD56 can be used to detect NK cells, γ/δ T cells and activated CD8+ cells.; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD45 is a single-chain type I transmembrane glycoprotein. Except for erythrocytes and platelets, CD45 is expressed on nearly all of the hematopoietic cells with high level. It is a common marker for blood leukocytes. CD45 is a receptor type protein tyrosine phophatase and plays essential roles in B cell and T cells signaling.

Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

5 uL/Test

FITC,PE,PE/Cyanine7,PerCP

For whole blood samples, add 5 uL Anti-Human CD19-FITC/CD56-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail to 100 uL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 uL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1x10^6 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 uL cell staining buffer and add 5 uL Anti-Human CD19-FITC/CD56-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 uL cell staining buffer and load the sample on flow cytometer for detection.