Elabscience

Lactate Dehydrogenase (LDH) Activity Assay Kit

Product Code:
 
E-BC-K046-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
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E-BC-K046-M-96T96T£164.00
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E-BC-K046-M-500Assays500Assays£334.00
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  • Further Information
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Further Information

Assay Time:
45 min
Detection Instrument:
Microplate reader
Detection Range:
6-1000 U/L
Detection Type:
Colorimetric method
Inter cv:
2.4
Intra cv:
1.8
Result Type:
Enzyme Activity
Sensitivity:
6 U/L
Target Synonym:
LDH
Test Principle:
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Tested Sample Types:
Serum,plasma,hydrothorax,tissue,cells

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0278691522002599
  • https://europepmc.org/article/med/34803688?javascript_support=no
  • https://pubs.rsc.org/en/content/articlelanding/2021/ra/d0ra08538c
  • https://www.hindawi.com/journals/ecam/2022/7832540/
  • https://www.spandidos-publications.com/10.3892/mmr.2016.5535?text=abstract
  • https://www.mdpi.com/2075-4426/13/4/649
  • https://mail.cellmolbiol.org/index.php/CMB/article/view/5014
  • https://www.nature.com/articles/s41598-024-64952-2
  • https://www.cell.com/heliyon/fulltext/S2405-8440(24)12196-2