B-Xpander™ CD40L (human) (rec.) (Animal Free)

MultimericCD40L™ (Animal Free); ACRP30headless:CD40L; ACRP30headless:CD154; ADIPOQ-CD40L; ACRP30headless:TNFSF5

Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).

After reconstitution: for 10ug size: 0.1mg/ml, for 100ug size: 1mg/ml

<0.01EU/ug purified protein (LAL test, Lonza).

Lyophilized. Contains PBS

Avoid freeze/thaw cycles.

CD40 ligand (CD40L) expressed by activated T cells engages CD40 expressed by B cells and APCs. CD40L can provide signals to B cells that induce proliferation, immunoglobulin class switching and antibody secretion. CD40L has been used recently in Immunotherapy to activate tumor-infiltrated lymphocytes (TIL). MultimericCD40L™ is a high-activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has been shown to be a potent tool for B cell expansion. It also has big potential as a growth factor for tumor-infiltrating lymphocytes (TILs), which have been shown to be important for T cell therapy. The CD40L (human) (multimeric) (rec.) (Animal Free) has been produced in CHO in suspension using animal-component free medium and certified CHO cells.

~35-40kDa (SDS-PAGE)

>95% (SDS-PAGE)

Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless?(aa 18-111) and a FLAG®-tag.

CHO cells. Produced using animal component-free medium.

Human

Fc (LALA-PG)-KIH (human) does not bind to FcRs.

Working aliquots are stable for up to 3 months when stored at -20°C.