Abnova Corporation

NIH/3T3 (mouse NIH/Swiss embryo) whole cell lysate (denatured)

Product Code:
L018V1
Host Type:
Mouse
Regulatory Status:
RUO
Application:
Western Blot (WB)
Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
 

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This product comes from: Taiwan.
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Further Information

Antigen Target Species:
Mouse
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Product Description:
Whole cell lysate (denatured)
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Recommended Dilutions:
The lysate is ready to load on SDS-PAGE for Western blotting.
Storage Buffer:
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Tissue:
Embryo, Contact-Inhibited