Mesothelial Cell Medium

Mesothelial Cell Medium (MesCM), when used with Mesothelial Cell Growth Supplement (MesCGS, Cat #5362) and 10 ml of fetal bovine serum (FBS) is a complete medium designed for normal mouse mesothelial cells in vitro . It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO 2 /95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal stellate cells in vitro .

MesCM consists of 500 ml of basal medium, 10 ml of FBS (Cat. #0010), 5 ml of Mesothelial Cell Growth Supplement (MesCGS, Cat. #5362) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, MesCGS and P/S are not pre-mixed in MesCM; they must be added separately to make the complete MesCM.