Ancell

Human IgG1 Isotype Control (APC)

Product Code:
 
ANC-295-060
Product Group:
 
Isotype Controls
Supplier:
 
Ancell
Host Type:
 
Human
Antibody Isotype:
 
Human IgG1κ
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
 
Blue Ice
Storage:
 
Short term: +4°C. Long term: +4°C
1 / 1
Binding of human IgG1kappa/APC to ICO-97-coated CML beads. Method: huIgG1kappa/APC was tested for binding to anti-human IgG (clone ICO-97)-coated 4u beads in FACS (Inquire). Approximately 5 x 10^5 beads per tube were spun down and incubated 45 min at 4°C with 80ul of huIgG1kappa/APC at a 1:50 dilution (10ug/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 3.45 log10 fluorescent units when compared to a buffer background. Binding was blocked when beads were pre-incubated 10 min with 20ul of 0.5 mg/ml unlabeled huIgG1kappa antibody (#ANC-295-010).

Binding of human IgG1kappa/APC to ICO-97-coated CML beads. Method: huIgG1kappa/APC was tested for binding to anti-human IgG (clone ICO-97)-coated 4u beads in FACS (Inquire). Approximately 5 x 10^5 beads per tube were spun down and incubated 45 min at 4°C with 80ul of huIgG1kappa/APC at a 1:50 dilution (10ug/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 3.45 log10 fluorescent units when compared to a buffer background. Binding was blocked when beads were pre-incubated 10 min with 20ul of 0.5 mg/ml unlabeled huIgG1kappa antibody (#ANC-295-010).

No additional charges, what you see is what you pay! *

CodeSizePrice
ANC-295-060-100ug100 ug£418.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Switzerland.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Concentration:
0.5mg/ml
Form (Short):
liquid
Formulation:
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Handling Advice:
Avoid freeze/thaw cycles. Protect from light.
Labels - Conjugates:
APC
Long Description:
Monoclonal Antibody. Isotype: Human IgG1kappa. Immunogen:Applications: ELISA, FACS. Human IgG1kappa from human myeloma plasma is used as a negative control for work involving human IgG1 isotype antibodies or recombinant proteins.
Package Type:
Plastic Vial
Product Description:
Human IgG1kappa from human myeloma plasma is used as a negative control for work involving human IgG1 isotype antibodies or recombinant proteins.
Purity:
Protein A purified.
Transportation:
Non-hazardous
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 6 months after receipt when stored at +4°C.

Documents