Elabscience

Rat AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) CLIA Kit

Product Code:
E-CL-R0517
Product Group:
CLIA Kits
Supplier:
Elabscience
Regulatory Status:
RUO
Target Species:
Rat
Application:
Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice bag
Storage:
2-8°C/-20°C, 6 months
1 / 1
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Assay Time:
3 h 30 min
Cross Reactivity:
This kit recognizes Rat AMPK in samples. No significant cross-reactivity or interference between Rat AMPK and analogues was observed
Detection Range:
0.63-40 ng/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.38 ng/mL
Target Species:
Rat
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AMPK. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AMPK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMPK, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat AMPK. You can calculate the concentration of Rat AMPK in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich-ELISA
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat AMPK concentrations in Serum, plasma and other biological fluids.

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