High-Density Lipoprotein Chlesterol (HDL-C) Fluorometric Assay Kit

40 min

Fluorescence microplate reader

0.15-295 umol/L

Fluorescence method

7.1-8.7%

2.1-2.4%

Quantitative

0.15 umol/L

High-density lipoprotein cholesterol (HDL-C), mainly synthesized in the liver, is an anti-atherosclerotic lipoprotein composed of apolipoprotein, phospholipid, cholesterol and fatty acids. The role of HDL-C is to transport phospholipids and cholesterol, which can transport cholesterol from extrahepatic tissues to the liver for metabolism, and be excreted from the body by bile acids. Plasma level of HDL-C are inversely associated with the risk of cardiovascular disease. HDL can take cholesterol from cell membranes, catalyze it into cholesterol esters by lecithin cholesterol acyltransferase, and then transfer the cholesterol ester to very low density lipoproteins and low density lipoproteins. The cholesterol content of high-density lipoprotein is relatively fixed, about 20-30% of the total cholesterol of the human body.
After a series of reactions of HDL-C, the product can form a complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured, and the content of HDL-C in the sample can be calculated.

Serum;Plasma;Animal tissue;Cell