Elabscience

Matrix Metalloproteinase 3 (MMP-3) Activity Fluorometric Assay Kit

Product Code:
 
E-BC-F061
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
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CodeSizePrice
E-BC-F061-48T48T£321.00
Quantity:
E-BC-F061-96T96T£417.00
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Further Information

Assay Time:
50 min
Detection Instrument:
Fluorescence Microplate reader
Detection Range:
0.03-9.66 U/L
Detection Type:
Fluorometric method
Inter cv:
7.5-7.7%
Intra cv:
2.0-4.0%
Result Type:
Enzyme Activity
Sensitivity:
0.03 U/L
Test Principle:
This kit is detected by fluorescence resonance energy transfer (FRET) method. MCA and Dnp are connected to two ends on the natural substrate of MMP-3 enzyme. When MMP-3 protease does not cut the substrate, the two groups are close enough to undergo fluorescence resonance energy transfer, that is, Dnp can quench the fluorescence of MCA, resulting in undetectable fluorescence. When the substrate is cut by MMP-3 protease, the ends and ends of the polypeptide are separated, the two groups are separated, the fluorescence of MCA is no longer extinguished by Dnp, and the fluorescence of MCA can be detected, so that the enzyme activity of MMP-3 protease can be detected very sensitively through fluorescence detection. MCA has a maximum excitation wavelength of 325 nm and a maximum emission wavelength of 393 nm.
Tested Sample Types:
serum;plasma;animal tissue;cell

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