Intracellular Acidification Rate Fluorometric Assay Kit

60 min

Fluorescence Microplate Reader

Fluorescence method

Quantitative

Intracellular acidification rate refers to the degree of change in the pH of the intracellular environment, which is an important parameter for the study of cell function. Constant intracellular pH level is an indispensable condition for maintaining normal cell metabolism and function. Most physiological and biochemical reactions in the cell, such as cell growth, calcium regulation, enzyme activity, receptor mediated signal transduction, ionic action, endocytosis, and cell adhesion, etc. Therefore, there is a close relationship between intracellular pH regulation and metabolism, and accurate detection of intracellular pH fluctuations is an important way to understand intracellular metabolism. At the same time, studying the interaction between intracellular pH regulation and metabolism will help to better understand the biochemical processes in cells and provide new ideas and methods for the treatment of related diseases. The probe used in this test box has the characteristics of dual excitation wavelength. The probe has the maximum absorption at 490 nm and an equal absorption point at 440 nm. By calculating the ratio of the two excitation wavelengths, the fluctuation of intracellular pH can be detected.

cell