Elabscience

Phospholipase D (PLD) Activity Colorimetric Assay Kit

Product Code:
 
E-BC-K783-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
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CodeSizePrice
E-BC-K783-M-48T48T£321.00
Quantity:
E-BC-K783-M-96T96T£417.00
Quantity:
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Further Information

Assay Time:
50 min
Detection Instrument:
Microplate reader
Detection Range:
0.68-16.73 U/L
Detection Type:
Colorimetric method
Inter cv:
1.8-9.5%
Intra cv:
1.7-2.8%
Result Type:
Enzyme activity
Sensitivity:
0.68 U/L
Test Principle:
Phospholipase D (PLD) exists widely in natural plants and animals, and PLD plays a key role in phospholipase metabolism. PLD hydrolyzes phosphodiester bonds of glycerol phospholipids to produce phosphatidylic acid and nitrogenous base. Abnormal PLD activity and expression are associated with Alzheimer's disease, stroke, cancer, and other brain diseases.
The detection principle of this kit is that PLD catalyze the substrate and the chromogenic agent to product colored substance, which has the maximum absorption at the wavelength of 550 nm. By measuring the OD value at 550 nm, PLD activity in the sample is calculated according to the standard curve.
Tested Sample Types:
Serum;Plasma;Animal and plant tissue

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