Elabscience

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

Product Code:
 
E-BC-K790-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
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CodeSizePrice
E-BC-K790-M-48T48T£348.00
Quantity:
E-BC-K790-M-96T96T£458.00
Quantity:
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Further Information

Assay Time:
50 min
Detection Instrument:
Microplate reader
Detection Range:
0.49-50.00 U/L
Detection Type:
Colorimetric method
Inter cv:
3.2-8.6%
Intra cv:
3.2-5%
Result Type:
Enzyme Activity
Sensitivity:
0.49 U/L
Target Synonym:
GAPDH
Test Principle:
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body. This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.
Tested Sample Types:
tissue;cell

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