Elabscience

NETosis Colorimetric Assay Kit

Product Code:
 
E-BC-K884-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
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CodeSizePrice
E-BC-K884-M-48T48T£266.00
Quantity:
E-BC-K884-M-96T96T£369.00
Quantity:
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This product comes from: China.
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Further Information

Assay Time:
7 h 20 min
Detection Instrument:
Microplate reader
Detection Range:
0.31-18.33 U/L
Detection Type:
Colorimetric method
Inter cv:
4.8-7.4%
Intra cv:
2.1-3.8%
Result Type:
Quantitative
Sensitivity:
0.31 U/L
Test Principle:
NETosis is an inflammatory cell death mode of neutrophils. Activated neutrophils capture and kill pathogens by releasing Neutrophil extracellular traps (NETs), which are composed of deaggregated chromatin and intracellular granule proteins. The formation of NETs is accompanied by the death of neutrophils. This new type of death is different from apoptosis and necrosis and is called NETosis. There are many inducers of NETosis, such as pathogens (bacteria, fungi, viruses, etc.), activated platelets, chemokines (interleukins, granulocyte colony-stimulating factor, transforming growth factor β, etc.), artificial ingredients and calcium ionophore, etc. These inducers can promote the key proteins of NETosis, Neutrophil Elastase (NE), Myeloperoxidase (MPO) and Peptidearginine Deaminase IV (PAD4) transcription and translation.
This method evaluated the degree of NETosis by detecting the enzyme activity of NE.
Tested Sample Types:
Cell

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