Ready-to-use 3D Human Renal Proximal Tubular Epithelial Spheroids - 96 plates

The kidney proximal tubule is a primary site of nephrotoxicity due to its frequent exposure to drugs and toxic compounds [1]. Recent studies have also shown that human renal tubule cells could be the potential host cells targeted by respiratory viruses such as SARS-CoV-2 [2]. Current pre-clinical models using 2D monolayer cultures and animal models are unable to fully recapitulate clinical drug response due to limited in vitro lifespan or species-specific variations. Hence 3D models of the human proximal tubular epithelial cells have been developed and are proven to maintain the differentiation hallmarks [3]. To serve as a relevant in vitro model and overcome the challenges of building 3D spheroids ScienCell has developed ready-to-use 3D renal proximal tub-ular epithelial spheroids (SP-HRPTEpiS). ScienCell's 3D spheroids display the differentiated epithelial cell marker CK18. Notably SP3D-HRPTEpiS also have high ACE2 (angiotensin-converting enzyme 2) expression a receptor for SARS-CoV and SARS-CoV-2. In addition our RT-PCR analysis indicate elevated mRNA expression of the renal proximal transport molecule aquaporin-1 AQP1 the organic anion transporters OAT 1/3 the organic cation transporter OCT 2 the multidrug and toxin extrusion proteins MATE 1/2 and P-glycoprotein P-gp in 3D spheroids compared to 2D monolayer. SP3D-HRPTEpiS therefore provide a physiological in vitro model to study kidney functions and immune response of kidney cells to viruses.

ScienCell's ready-to-use 3D renal proximal tubular epithelial spheroids (SP-HRPTEpiS) display the differentiated epithelial cell marker CK18. Notably SP3D-HRPTEpiS also have high ACE2 (angiotensin-converting enzyme 2) expression. Our RT-PCR analysis indicate elevated mRNA expression of the renal proximal transport molecule and transporters in 3D spheroids compared to 2D monolayer. SP3D-HRPTEpiS therefore provide a physiological in vitro model to study kidney functions and immune response of kidney cells to viruses.