Ready-to-use 3D Human Chondrocyte-articular Spheroid Kit - 96 plates

The composition and structural integrity of extracellular matrix (ECM) within the cartilage is responsible for enduring the tensile strength of joint biomechanics. Chondrocytes the main constituent of cartilage mediate the synthesis and degradation of ECM macromolecules (such as Type II collagen and aggrecan) within the matrix. Type II collagen and aggrecan provide the tensile strength and the osmotic resistance for cartilage respectively. Chondrocytes in monolayer culture however are susceptible to dedifferentiation. Thus an improved cell culture model that closely mimics the in vivo environment is necessary to maintain the features of differentiated chondrocytes. ScienCell has developed a highly innovative ready-to-use 3D chondrocyte spheroid kit (SP3D-HCaS) that better approximates the in vivo environment. Using ScienCell's Ready-to-use 3D spheroid kit researchers can obtain highly homogenous and functional 3D spheroids in 24 hours after thawing without encountering the long and complex workflow of 3D culture. In 3D spheroid culture we confirmed that the chondrocytes maintain functional markers such as type II collagen aggrecan and Sox9 as determined by qPCR analysis. ScienCell's SP3D-HCaS provides an excellentin vitromodel for studying normal chondrocyte physiology mechanism of degenerative joint diseases and cartilage tissue repair and engineering.

Ready-to-use 3D human chondrocyte-articular spheroid kit (SP3D-HCaS) is an innovative and convenient 3D culture product. In 24 hours after thawing the cryopreserved 3D spheroids researchers can obtain highly functional and homogenous 3D chondrocyte spheroids.