KactusBio Inc.

Chimeric HLA-A*02:01 (ma3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM150)

Product Code:
 
KAC-MHC-HM150
Product Group:
 
MHC Monomers
Supplier:
 
KactusBio Inc.
Regulatory Status:
 
RUO
Shipping:
 
Shipped with dry ice.
Storage:
 
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag with an affinity constant of 67.84 nM as determined in SPR assay (Biacore T200)
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Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test)
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Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
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The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC.

Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag with an affinity constant of 67.84 nM as determined in SPR assay (Biacore T200)
Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test)
Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC.

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CodeSizePrice
KAC-MHC-HM150-100UG100UG£414.00
Quantity:
KAC-MHC-HM150-500UG500UG£1,061.00
Quantity:
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Background:
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of? the MHC Class I, B2M and YLEPGPVTA peptide of? the gp100.
Biotinylated:
no
Description:
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide.
Endotoxin:
Less than 1EU per ug by the LAL method.
Exact Sequence:
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide
Expression System:
HEK293
Form:
Liquid
Formulation:
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Gene/Protein ID:
A0A140T933(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&YLEPGPVTA
Molecular Weight:
The protein has a predicted MW of 47.90 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Purity:
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Tag:
C-His
Target:
HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer

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