KactusBio Inc.

Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453B)

Product Code:
 
KAC-MHC-MM453B
Product Group:
 
MHC Monomers
Supplier:
 
KactusBio Inc.
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Shipping:
 
Shipped at ambient temperature.
Storage:
 
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
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The purity of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is greater than 95% as determined by SEC-HPLC.
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Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is greater than 95% as determined by SEC-HPLC.

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CodeSizePrice
KAC-MHC-MM453B-100UG100UG£327.00
Quantity:
KAC-MHC-MM453B-500UG500UG£1,073.00
Quantity:
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Background:
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Biotinylated:
yes
Description:
Recombinant Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide.
Endotoxin:
Less than 1EU per ug by the LAL method.
Exact Sequence:
His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide
Expression System:
HEK293
Form:
Lyophilized
Formulation:
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Gene/Protein ID:
P01901(H-2K(b))&P01887(B2M)&SIINFEKL
Molecular Weight:
The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Purity:
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Reconstitution:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Tag:
C-His-Avi
Target:
H-2K(b)&B2M&OVA (SIINFEKL) Monomer

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