KactusBio Inc.

Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein (HLG-RM41CB)

Product Code:
 
KAC-HLG-RM41CB
Product Group:
 
MHC Monomers
Supplier:
 
KactusBio Inc.
Regulatory Status:
 
RUO
Shipping:
 
Shipped at ambient temperature.
Storage:
 
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Immobilized Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL), His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA
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Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
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The purity of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC.
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Immobilized Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL), His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA
Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC.

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Further Information

Background:
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Biotinylated:
yes
Description:
Recombinant Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide.
Endotoxin:
Less than 1EU per ug by the LAL method.
Exact Sequence:
Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
Expression System:
HEK293
Form:
Lyophilized
Formulation:
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Gene/Protein ID:
O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL
Molecular Weight:
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Purity:
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Reconstitution:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Tag:
C-His-Avi
Target:
HLA-G&B2M&Peptide (RIIPRHLQL) Monomer

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