KactusBio Inc.

Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer?Protein (MHC-HM43RB)

Product Code:
 
KAC-MHC-HM43RB
Product Group:
 
MHC Monomers
Supplier:
 
KactusBio Inc.
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Shipped at ambient temperature.
Storage:
 
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA
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Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
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The purity of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 90% as determined by SEC-HPLC.
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Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA
Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 90% as determined by SEC-HPLC.

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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Background:
HLA-A*02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Biotinylated:
yes
Description:
Recombinant Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01) and Ile21-Met119(B2M).
Endotoxin:
Less than 1EU per ug by the LAL method.
Exact Sequence:
Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Expression System:
HEK293
Form:
Lyophilized
Formulation:
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Gene/Protein ID:
A0A140T913(HLA-A*02:01)&P61769(B2M)
Molecular Weight:
The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Purity:
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Reconstitution:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Tag:
C-His-Avi
Target:
Peptide Ready HLA-A*02:01&B2M Monomer

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