mRNA Cap 2'-O-Methyltransferase GMP-Grade (GMP-MEH-VE101) DMF #038029

mRNA Cap 2'-O-Methyltransferase, utilizing S-adenosylmethionine (SAM) as a methyl group donor, adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure. Cap1 structure is a signature of self RNA.and has been shown to modulate binding of innate immune sensors. For instance, the binding affinity of IFIT-1 for Cap1 is much weaker than for 5' triphosphate or Cap0 structure; Cap1 structure abrogates RIG-1 signaling and prevents detection by MDA5.

NOTE: Vaccinia Capping Enzyme #GMP-VCS-VE101) is required to generate Cap0 structure.

This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038029.

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