Abnova Corporation

293T whole cell lysate (denatured)

Product Code:
 
L151V1
Supplier:
 
Abnova Corporation
Host Type:
 
HEK 293 cells
Regulatory Status:
 
RUO
Application:
 
Western Blot (WB)
Storage:
 
Store at -20C or lower. Aliquot to avoid repeated freezing and thawing.
 

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This product comes from: Taiwan.
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Further Information

Description:
Whole cell lysate (denatured).
Lysis Buffer:
1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Preparation Method:
Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90C for 10 minutes, and then cooled rapidly.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Raised in Host Species:
Human
Recommend Dilutions:
The lysate is ready to load on SDS-PAGE for Western blotting.
Storage Buffer:
In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Tissue:
Kidney