Abnova Corporation

CHD4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Product Code:
 
L034T6
Supplier:
 
Abnova Corporation
Host Type:
 
HEK 293 cells
Regulatory Status:
 
RUO
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Store at -80C. Aliquot to avoid repeated freezing and thawing.
 

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Further Information

Concentration:
2 mg/ml
Genbank Accession:
NM_001273
Gene Alias:
DKFZp686E06161|Mi-2b|Mi2-BETA
Gene Description:
chromodomain helicase DNA binding protein 4
Gene Name:
CHD4
Geneid:
1108
Host Cell:
HEK293
Lysis Buffer:
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Plasmid:
pCMV-CHD4 full length
Protein Accession:
NP_001264
Quality Control Testing:
Transient overexpression cell lysate was tested with Anti-CHD4 antibody (H00001108-M01) by Western Blots.
Raised in Host Species:
Human
Recommend Dilutions:
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer:
In modified RIPA Lysis Buffer.