Elabscience

Histone Deacetylase (HDAC) Activity Fluorometric Assay Kit

Product Code:
 
E-BC-F051
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
 

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E-BC-F051-96T96T£458.00
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Further Information

Assay Time:
1 h 30 min
Detection Instrument:
Fluorescence microplate reader
Detection Range:
0.019-1.027 U/L
Detection Type:
Fluorometric method
Inter cv:
6.9-8.8%
Intra cv:
3.3-4.6%
Result Type:
Enzyme activity
Sensitivity:
0.019 U/L
Test Principle:
Histone deacetylases (HDAC) are a type of protease that play an important role in the structural modification of chromosomes and the regulation of gene expression. Earlier studies have shown that HDAC has a close relationship with tumors. The overexpression of HDAC can lead to various types of cancer as well as other neurological diseases, autoimmune diseases, inflammatory diseases, heart and lung diseases.
Detection principle: HDAC specifically cleaves the fluorescent substrate, and the fluorescent group emits fluorescence which is detected at the excitation wavelength of 355 nm and the emission wavelength of 460 nm. Specific inhibitors are used in the kit to inhibit enzyme activity and eliminate the influence of non-specific enzyme activity.
Tested Sample Types:
Serum;Plasma;Animal tissue;Cell

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