Elabscience

β-1,3-Glucanase Activity Colorimetric Assay Kit

Product Code:
 
E-BC-K1223-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at 2-8°C for 12 months with shading light.
 

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E-BC-K1223-M-96T96T£348.00
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Further Information

Assay Time:
50 min
Detection Instrument:
Microplate reader
Detection Range:
6.22-333.33 U/mL
Detection Type:
Colorimetric method
Inter cv:
2.6-3.8%
Intra cv:
4.1-6.4%
Result Type:
Enzyme activity
Sensitivity:
15.33 U/mL
Test Principle:
β-1, 3-glucanase (β-1,3-GA, EC 3.2.1.39) is mainly found in plants and catalyzes the hydrolysis of β-1, 3-glucosidic bonds, thereby destroying fungal cell walls. In particular, when synergistic with chitinase, it can significantly inhibit the growth of fungi. When plants are infected with diseases or under other stress conditions, they can induce a large amount of β-1, 3-GA to enhance plant resistance to adverse external stimuli. Therefore, the determination of β-1, 3-GA activity is widely used in plant pathology and stress physiology research.
β-1,3-GA hydrolyses the β-1, 3-glucosidic linkage of laminarin to produce reducing ends. The activity can be reflected by measuring the amount of reducing sugar using 3, 5-dinitrosalicylic acid.
Tested Sample Types:
Plant tissue

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