Elabscience

Low-density Lipoprotein Cholesterol(LDL-C) Assay Kit

Product Code:
 
E-BC-K205-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at 2-8°C for 12 months with shading light.
 

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CodeSizePrice
E-BC-K205-M-96T96T£164.00
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This product comes from: China.
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  • Further Information
  • Documents
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Further Information

Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.04-12 mmol/L
Detection Type:
Colorimetric method
Inter cv:
0.001
Intra cv:
0.00055
Result Type:
Quantitative
Sensitivity:
0.04 mmol/L
Test Principle:
Lipoproteins (except LDL) such as HDL, CM, and VLDL change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. Because the cholesterol molecules of other lipoproteins have been removed, the shade is proportional to the amount of LDL-C.
Tested Sample Types:
Serum;Plasma;Cell

Documents

References

  • https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvae148/7717207
  • https://www.sciencedirect.com/science/article/pii/S2212877824001844
  • https://www.tandfonline.com/doi/abs/10.2147/IJN.S478589
  • https://www.sciencedirect.com/science/article/pii/S0944711324005440
  • https://www.sciencedirect.com/science/article/pii/S094471132400432X
  • https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo03183k
  • https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c03172
  • https://www.sciencedirect.com/science/article/pii/S0014299923007756
  • https://www.mdpi.com/2304-8158/13/17/2760
  • https://www.sciencedirect.com/science/article/pii/S1567576924011901
  • https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400412RR
  • https://www.sciencedirect.com/science/article/pii/S2212429224001755