High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

30 min

Microplate reader

0.06-3.8 mmol/L

Colorimetric method

0.0005

0.0003

Quantitative

0.06 mmol/L

CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound,the shade is proportional to the amount of HDL-C.

Serum;Plasma