Elabscience

S-Adenosyl-Homocysteine Hydrolase (SAHH) Activity Colorimetric Assay Kit

Product Code:
 
E-BC-K841-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
 

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CodeSizePrice
E-BC-K841-M-96T96T£348.00
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Further Information

Assay Time:
1 h
Detection Instrument:
Microplate reader
Detection Range:
1.34-37.14 U/L
Detection Type:
Colorimetric method
Inter cv:
3.7-7.8%
Intra cv:
1.8-3.4%
Result Type:
Enzyme activity
Sensitivity:
1.34 U/L
Test Principle:
In mammalian cells, S-adenosine homocysteine hydrolase (SAHH) is the only hydrolase involved in the hydrolysis of S-adenosine homocysteine (SAH) to homocysteine (Hcy). Excessive SAHH activity can hydrolyze SAH to generate excessive Hcy, thereby causing geriatric diseases such as Alzheimer's disease, hypercysteinemia, and coronary heart disease. Inhibiting SAHH activity can effectively alleviate the hydrolysis of SAH.
The detection principle of this kit: The substrate is hydrolyzed by SAHH, and the product reacts with the chromogenic agent at room temperature to form a yellow colored product, which has the maximum absorption peak at 410 nm. The activity of SAHH can be calculated by detecting the change in absorbance at 410 nm.
Tested Sample Types:
Animal tissue;Cell

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