Elabscience

Chymotrypsin Activity Colorimetric Assay Kit

Product Code:
 
E-BC-K845-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.
 

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CodeSizePrice
E-BC-K845-M-48T48T£252.00
Quantity:
E-BC-K845-M-96T96T£348.00
Quantity:
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Further Information

Assay Time:
1 h 10 min
Detection Instrument:
Microplate reader
Detection Range:
0.028-1.01 U/L
Detection Type:
Colorimetric method
Inter cv:
1.2-9.7%
Intra cv:
1.8-4.0%
Result Type:
Enzyme activity
Sensitivity:
0.028 U/L
Test Principle:
Chymotrypsin is a serine trypsin secreted by the pancreas, which is mainly used for the decomposition of proteins during digestion. It specifically hydrolyzes peptide bonds of aromatic amino acids (such as phenylalanine, tryptophan, and tyrosine) in proteins to generate smaller peptides or amino acids for further absorption by the body. In addition, chymotrypsin is widely used in protein analysis and research in the laboratory, especially for protein sequencing and structural analysis.
The detection principle of this kit is: The substrate was converted to a colored substance after chymotrypsin catalysis, and the OD value was detected at a wavelength of 405 nm, and the enzyme activity was calculated from the OD value of the standard.
Tested Sample Types:
Tissue

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