Elabscience

Dehydroascorbic Acid Colorimetric Assay Kit

Product Code:
 
E-BC-K864-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice bag
Storage:
 
This product can be stored at 2-8°C for 12 months with shading light.
 

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CodeSizePrice
E-BC-K864-M-96T96T£307.00
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This product comes from: China.
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Further Information

Assay Time:
40 min
Detection Instrument:
Microplate reader
Detection Range:
0.03-1.00 umol/mL
Detection Type:
Colorimetric method
Inter cv:
2.5-4.2%
Intra cv:
3.5-5.6%
Result Type:
Quantitative
Sensitivity:
0.0016 umol/mL
Test Principle:
Ascorbic acid (AsA) is an important physiological index of plant cells. The changes of its content, redox status (AsA/DHA ratio), and the activities of enzymes related to its synthesis and metabolism are involved in the response of plants to a series of environmental stresses. Dehydroascorbic acid (DHA) is a reversible oxidized form of AsA. In vivo, dehydroascorbic acid (DHA) and ascorbic acid form a redox system and act as an electron acceptor.
The detection principle of this kit is that DTT reduces DHA to produce AsA, and the DHA content can be calculated by measuring the formation rate of AsA in the system at 265 nm.
Tested Sample Types:
Plant tissue

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