IL-18 (mouse) (monomer):Fc (silent) InVivoKine™

After reconstitution: 1mg/ml

IL-18, initially named interferon-gamma-inducing factor (IGIF) is an IL-1 family member and a cytokine that links innate and adaptive immunity. Depending on the microenvironment and timing, IL-18 can either drive harmful inflammation or restore immune homeostasis. IL-18 is central to host defense mechanisms, acting on T helper cells, NK cells, NKT cells, B cells, and dendritic cells to promote the production of IFN-gamma, a critical mediator of immune responses. IL-18 is produced mainly by macrophages, but it is also found in barrier epithelial cells of the skin, upper airway and gastrointestinal tracts. IL-18 is regulated i) by the inflammasome that converts the inactive pro-IL-18 to its active form IL-18; ii) by the cytokine it induces, the master cytokine IFN-gamma that triggers the expression of the inhibitor of IL-18, called IL-18 binding protein (IL-18BP). The IL-18 (mouse) (monomer):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the IL-18 (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 48kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK 293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996) required for dimerization of the Fc moieties and for secretion of the final protein IL-18 (mouse) (monomer):Fc (silent) InVivoKine™. The LALA-PG mutations inhibit binding to FcgammaRs and C1q, while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical, and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

32160000

<0.01EU/ug protein (LAL test).

solid

Lyophilized. Contains PBS.

After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0. 1% BSA should be used for further dilutions.

InVivoKines™. Recombinant Protein. Mouse IL-18 (aa 36-192) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). Produced in HEK 293 cells. Produced using animal component-free medium. IL-18, initially named interferon-gamma-inducing factor (IGIF) is an IL-1 family member and a cytokine that links innate and adaptive immunity. Depending on the microenvironment and timing, IL-18 can either drive harmful inflammation or restore immune homeostasis. IL-18 is central to host defense mechanisms, acting on T helper cells, NK cells, NKT cells, B cells, and dendritic cells to promote the production of IFN-gamma, a critical mediator of immune responses. IL-18 is produced mainly by macrophages, but it is also found in barrier epithelial cells of the skin, upper airway and gastrointestinal tracts. IL-18 is regulated i) by the inflammasome that converts the inactive pro-IL-18 to its active form IL-18; ii) by the cytokine it induces, the master cytokine IFN-gamma that triggers the expression of the inhibitor of IL-18, called IL-18 binding protein (IL-18BP). The IL-18 (mouse) (monomer):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the IL-18 (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 48kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK 293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996) required for dimerization of the Fc moieties and for secretion of the final protein IL-18 (mouse) (monomer):Fc (silent) InVivoKine™. The LALA-PG mutations inhibit binding to FcgammaRs and C1q, while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical, and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

~48kDa and 28kDa (SDS-PAGE)

P70380

Plastic Vial

>95% (SDS-PAGE)

Mouse IL-18 (aa 36-192) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).

Fc

Non-hazardous

Interleukins

41116127

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.