UBE3A (E3 Ligase) (human) (rec.) (His)
Product Code:
SBB-CE0090
SBB-CE0090
Regulatory Status:
RUO
RUO
Target Species:
Human
Human
Shipping:
Dry Ice
Dry Ice
Storage:
Short term: -80°C. Long term: -80°C
Short term: -80°C. Long term: -80°C
No additional charges, what you see is what you pay! *
| Code | Size | Price |
|---|
| SBB-CE0090-C050 | 50 ug | £376.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
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Further Information
Alternate Names/Synonyms:
Ubiquitin-protein Ligase E3A; E6AP Ubiquitin-protein Ligase; HECT-type Ubiquitin Transferase E3A; Human Papillomavirus E6-associated Protein; Oncogenic Protein-associated Protein E6-AP
Concentration:
Lot dependent.
EClass:
32160000
Form:
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.3, 100mM NaCl, 100uM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Recombinant Protein. Recombinant human UBE3A (Accession Nr. Q05086) has a C-terminal 8x His-Tag. Produced in Sf9 cells. Liquid. In 50mM HEPES pH 7.3, 100mM NaCl, 100uM TCEP. UBE3A (Ubiquitin-protein ligase E3A) is a 101 kDa E3 Ligase enzyme. The protein contains a C4 type zinc-finger and belongs to the HECT-type E3 ligase family with its active site cystein located at aa 843. UBE3A is involved in multiple diseases. It is used by the oncoprotein E6 (HPV) to trigger p53 degradation, enabling cervical cancer. It appears to be involved in metastatic prostate cancer, linked to Angelman Syndrome, and elevated expression is correlated with autism disorders. Furthermore, recent findings revealed a high-affinity binding site RAZUL (Rpn10 AZUL-binding domain), with the proteasome and with Rpn10 (PSMD4), prompting its involvement in the ubiquitination of many proteins destined for 26S proteasome degradation. Knockdown of UBE3A leads to a significant reduction in ubiquitinated proteins at the proteasome. It is hypothesized that UBE3A may belong to a subset of E3 ligases prioritized by the proteasome. This recombinant protein (human) has been expressed and purified from Sf9 insect cells and has a C-terminal 8x His-Tag.
NCBI, Uniprot Number:
Q05086
Package Type:
Plastic Vial
Product Description:
UBE3A (Ubiquitin-protein ligase E3A) is a 101 kDa E3 Ligase enzyme. The protein contains a C4 type zinc-finger and belongs to the HECT-type E3 ligase family with its active site cystein located at aa 843. UBE3A is involved in multiple diseases. It is used by the oncoprotein E6 (HPV) to trigger p53 degradation, enabling cervical cancer. It appears to be involved in metastatic prostate cancer, linked to Angelman Syndrome, and elevated expression is correlated with autism disorders. Furthermore, recent findings revealed a high-affinity binding site RAZUL (Rpn10 AZUL-binding domain), with the proteasome and with Rpn10 (PSMD4), prompting its involvement in the ubiquitination of many proteins destined for 26S proteasome degradation. Knockdown of UBE3A leads to a significant reduction in ubiquitinated proteins at the proteasome. It is hypothesized that UBE3A may belong to a subset of E3 ligases prioritized by the proteasome. This recombinant protein (human) has been expressed and purified from Sf9 insect cells and has a C-terminal 8x His-Tag.
Purity:
>98% (SDS-PAGE)
Sequence:
Recombinant human UBE3A (Accession Nr. Q05086) has a C-terminal 8x His-Tag.
Source / Host:
Sf9 cells
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.