South Bay Bio

DDB1/CRBN-Complex (human) (rec.) (untagged)

Product Code:
 
SBB-SC0116
Product Group:
 
Recombinant Proteins
Supplier:
 
South Bay Bio
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Dry Ice
Storage:
 
Short term: -80°C. Long term: -80°C
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DDB1/CRBN SDS-PAGE. From left to right, increasing amounts of DDB1/CRBN were loaded onto a 4-20% SDS-PAGE gel, stained with Coomassie brillant blue. Purity is >98%.
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DDB1/CRBN SDS-PAGE. From left to right, increasing amounts of DDB1/CRBN were loaded onto a 4-20% SDS-PAGE gel, stained with Coomassie brillant blue. Purity is >98%.

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CodeSizePrice
SBB-SC0116-C02525 ug£376.00
Quantity:
SBB-SC0116-C05050 ug£494.00
Quantity:
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
DNA Damage-binding Protein 1/Cereblon
Concentration:
Lot dependent.
EClass:
32160000
Form:
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol, 1mM TCEP
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Recombinant Protein. Recombinant human DDB1 (1-1140) with CRBN (2-442) complex is untagged. Produced in Sf9 cells. Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol, 1mM TCEP Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediates the ubiquitination of numerous target proteins, such as ILF2 and MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. In vivo, the endogenous substrate of CRBN MEIS2 is blocked from binding by immunomodulatory drugs (IMiDs), i.e. Thalidomide, which instead promotes the recruitment of IKZF1 or IKZF3 for degradation. DDB1 and CRBN complex has been coexpressed and purified from insect cells Sf9.
NCBI, Uniprot Number:
Q16531: DDB1 / Q96SW2: CRBN
Package Type:
Plastic Vial
Product Description:
Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediates the ubiquitination of numerous target proteins, such as ILF2 and MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. In vivo, the endogenous substrate of CRBN MEIS2 is blocked from binding by immunomodulatory drugs (IMiDs), i.e. Thalidomide, which instead promotes the recruitment of IKZF1 or IKZF3 for degradation. DDB1 and CRBN complex has been coexpressed and purified from insect cells Sf9.
Purity:
>98% (SDS-PAGE)
Sequence:
Recombinant human DDB1 (1-1140) with CRBN (2-442) complex is untagged.
Source / Host:
Sf9 cells
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.

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