RF-Absorbent

SERION Rheumatoid Factor (Rf)-Absorbent for IgM-Antibody-Detection - Anti- human- IgG for the removal of IgM- rheumatoid factors from serum- and plasmaprobes or cerebrospinal fluid (CSF) which are to be prepared for the detection of specific IgM-antibodies in indirect Immunoassays.
Principle of Method
Rheumatoid factors are autoantibodies (anti-IgG) mainly of the IgM-class. They can be detected in the serum of approx. 5 % of healthy individuals. IgM-rheumatoid factors preferably bind to IgG-immune-complexes; therefore, false positive results may appear in the pathogen-specific IgM-detection. Furthermore, the possibility exists, that weakbinding pathogen-specific IgM antibodies are displaced by stronger-binding IgG antibodies. In this case, IgM-detection can bring about false negative results. In IgAdetection possibly occuring competition with IgG-antibodies can also be prevented by eliminating IgG with Rf-absorbent. The SERION Rf-absorbent precipitates up to 15 mg IgG/ml (undiluted serum). The IgMrheumatoid factor binds to the precipitate. Consequently, the IgG-antibodies as well as the IgM-rheumatoid factors are not reactive in the immunoassay. The use of SERION rheumatoid factor-absorbent can minimize false positive as well as false negative results.
For Research Use Only