Elabscience

Human Granzyme B Activity Detection Substrate for Flow Cytometry

Product Code:
E-CK-A480
Supplier:
Elabscience
Regulatory Status:
RUO
Shipping:
Ice bag
Storage:
This product can be stored at -20°C for 1 year in the dark.
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Jurkat cells were?stained?with Human Granzyme B Substrates?(Green) and?analyzed?by?flow?cytometry.
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Jurkat cells were?stained?with Human Granzyme B Substrates?(Green) and?analyzed?by?flow?cytometry.

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Assay Time:
30 min
Detection Instrument:
Flow Cytometer
Detection Type:
Flow cytometry
Expiry date:
12 months
Kit Type:
Cell function detection kit
Test Principle:
Human Granzyme B Substrate (Green) is generated by conjugating a Granzyme B-specific recognition peptide (sequence IEPD) with a high-affinity DNA fluorescent dye. The substrate is cell-membrane-permeable and can penetrate the plasma membrane into the cytoplasm. In living cells without active Granzyme B, charge repulsion occurs between the substrate and DNA, resulting in no cellular fluorescence signal. In contrast, in living cells with active Granzyme B, the activated Granzyme B cleaves the substrate and releases the high-affinity DNA dye. The released DNA dye binds to intracellular DNA and emits green fluorescence, thereby enabling the detection of Granzyme B activity.
Tested Sample Types:
Cell samples

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