Elabscience

Citrate Fluorometric Assay Kit

Product Code:
E-BC-F153
Product Group:
Biochemical Assay Kits
Supplier:
Elabscience
Regulatory Status:
RUO
Shipping:
Ice bag
Storage:
This product can be stored at -20°C for 12 months with shading light.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Assay Time:
1 h 30 min
Detection Instrument:
Fluorescence microplate reader
Detection Range:
0.81-100 umol/L
Detection Type:
Fluorometric method
Expiry date:
12 months
Inter cv:
1.1%-5.8%
Intra cv:
0.8%-1.3%
Result Type:
Quantitative
Sensitivity:
0.81 umol/L
Test Principle:
Citrate is widely present in animal blood, tissues, and plant fruits. In living organisms, citrate is typically synthesized from oxaloacetate and acetyl-CoA via citrate synthase. As an intermediate metabolite in the tricarboxylic acid (TCA) cycle, CA can be rapidly utilized to generate ATP under stress conditions, thereby enhancing the organism's resistance to stress.
Citrate is converted into oxaloacetate under the catalysis of citrate lyase. Oxaloacetate is subsequently transformed into hydrogen peroxide (H2O2) via multiple enzymatic reactions. The generated H2O2 reacts with the fluorescent probe ADHP to produce a fluorescent substance. The fluorescence intensity can be measured to calculate the citrate content in the sample.
Tested Sample Types:
Serum, Plasma, Animal tissue, Plant tissue, Cell

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