Elabscience

NADPH Fluorometric Assay Kit

Product Code:
E-BC-F170
Product Group:
Biochemical Assay Kits
Supplier:
Elabscience
Regulatory Status:
RUO
Shipping:
Ice bag
Storage:
This product can be stored at -20°C for 12 months with shading light.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Assay Time:
2 h
Detection Instrument:
Fluorescence microplate reader
Detection Range:
8.55-1000 nmol/L
Detection Type:
Fluorometric method
Expiry date:
12 months
Inter cv:
3.3%-8.4%
Intra cv:
1.7%-5.0%
Result Type:
Quantitative
Sensitivity:
8.55 nmol/L
Test Principle:
Glucose-6-phosphate (G6P) is oxidized by glucose-6-phosphate dehydrogenase (G6PDH) to gluconolactone-6-phosphate (6-PG). At the same time, NADP+ is reduced to NADPH in this reaction. The generated NADPH reacts with a chromogenic agent under enzymatic catalysis to produce a fluorescent substance. The fluorescence intensity can be measured to calculate the NADPH content in the sample.
Tested Sample Types:
Animal tissue, Cell

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