CellaQuant™ Human IFN-B (Interferon Beta) ELISA Kit

3 h 30 min

Interferon-beta (IFN-β), a type I interferon, plays a key role in innate immunity against infections, tumors and inflammatory stimuli. Produced by fibroblasts, dendritic cells, macrophages and endothelial cells, it exerts diverse functions via specific signaling pathways and is clinically used to treat multiple sclerosis (MS). IFN-β mainly signals by binding to the heterodimeric receptor (high-affinity IFNAR2 and low-affinity IFNAR1), activating the Jak-STAT pathway. This regulates interferon-responsive genes encoding antiviral, proliferation-regulating and immunoregulatory proteins. It can also function via IFNAR1 alone, independent of Jak-STAT, enhancing its signaling versatility.Its biological effects include antiviral/antibacterial activities, regulation of B-cell development, myelopoiesis and LPS-inducible tumor necrosis factor. Notably, it is more potent than IFN-α in inducing apoptotic and antiproliferative pathways for tumor control, and protects dopaminergic neurons by maintaining neuronal homeostasis.

This kit recognizes Human IFN-β in samples.No significant cross-reactivity or interference between and analogues was observed

7.81-500 pg/mL

Colorimetric method, ELISA, Sandwich

12 months

3456

Both intra-CV and inter-CV are < 10%.

80%-120%

4.69 pg/mL

IFN-beta, IFNB1, IFB, IFNB, Interferon beta, Fibroblast interferon

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 17 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

Cell culture supernatant, Cell lysates or other biological fluids

Sandwich-ELISA

P01574

This ELISA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in cell culture supernatant, cell lysates or other biological fluids.