Human MCSFR(Macrophage Colony Stimulating Factor Receptor) ELISA Kit

3 h 30 min

M-CSF Receptor (M-CSF R), the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. M-CSF Receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta. Human M-CSF Receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF Receptor, releasing the functional ligand-binding extracellular domain. M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction. The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals. M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. M-CSF R and Integrin? alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis . In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear.

This kit recognizes Human MCSFR in samples. No significant cross-reactivity or interference between Human MCSFR and analogues was observed

62.5-4000 pg/mL

Colorimetric method, ELISA, Sandwich

12 months

1436

Both intra-CV and inter-CV are < 10%.

80%-120%

37.5 pg/mL

CSF1R, Colony stimulating factor 1 receptor, CD115, C-FMS, CSF-1R, FIM2, FMS, M-CSFR

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCSFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCSFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCSFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human MCSFR. You can calculate the concentration of Human MCSFR in the samples by comparing the OD of the samples to the standard curve.

Serum, plasma and other biological fluids

Sandwich-ELISA

P07333

This ELISA kit applies to the in vitro quantitative determination of Human MCSFR concentrations in Serum, plasma and other biological fluids.