Human FcγR II/CD32(Receptor II for the Fc Fragment of IgG) ELISA Kit
Product Code:
E-EL-H1743
E-EL-H1743
Regulatory Status:
RUO
RUO
Target Species:
Human
Human
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice bag
Ice bag
Storage:
2-8°C
2-8°C
No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Show All
Further Information
Assay Time:
3 h 30 min
Background:
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Cross Reactivity:
This kit recognizes Human FcγRII/CD32 in samples. No significant cross-reactivity or interference between Human FcγRII/CD32 and analogues was observed
Detection Range:
46.88-3000 pg/mL
Detection Type:
Colorimetric method, ELISA, Sandwich
Expiry date:
12 months
GeneID:
2213
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
28.13 pg/mL
Target Synonym:
Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FcγRII/CD32. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcγRII/CD32 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcγRII/CD32, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human FcγRII/CD32. You can calculate the concentration of Human FcγRII/CD32 in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich-ELISA
UNIProt ID:
P31994
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human FcγRII/CD32 concentrations in Serum, plasma and other biological fluids.