Mouse CD30L(Cluster of Differentiation 30 Ligand) ELISA Kit
Product Code:
E-EL-M0297
E-EL-M0297
Regulatory Status:
RUO
RUO
Target Species:
Mouse
Mouse
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice bag
Ice bag
Storage:
2-8°C
2-8°C
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This product comes from: China.
Typical lead time: 14-21 working days.
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Typical lead time: 14-21 working days.
Contact us for more accurate information.
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Further Information
Assay Time:
3 h 30 min
Background:
CD30 Ligand is a B cell surface antigen and a ligand for CD30 (TNFRSF8), playing an inhibitory role in CD40-mediated immunoglobulin class switching. CD30 Ligand is also a a marker for Hodgkin lymphoma and related hematologic malignancies, involves in activation and functioning of the T cell-dependent immune system. CD30 ligand is expressed by T and B lymphocytes, macrophages, and a variety of normal haematopoietic cells and derived tumours. CD30 ligand exerts pleiotropic effects on normal and malignant lymphoid cells, including death, differentiation, or cell division.
Cross Reactivity:
This kit recognizes Mouse CD30L in samples. No significant cross-reactivity or interference between Mouse CD30L and analogues was observed
Detection Range:
0.16-10 ng/mL
Detection Type:
Colorimetric method, ELISA, Sandwich
Expiry date:
12 months
GeneID:
21949
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.1 ng/mL
Target Synonym:
CD30 ligand, Tumor necrosis factor ligand superfamily member 8, CD153, Tnfsf8, Cd30l, Cd30lg,
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD30L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD30L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD30L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Mouse CD30L. You can calculate the concentration of Mouse CD30L in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich-ELISA
UNIProt ID:
P32972
Usage:
This ELISA kit applies to the in vitro quantitative determination of Mouse CD30L concentrations in Serum, plasma and other biological fluids.