Mini Sample Mouse GFAP (glial fibrillary acidic protein) ELISA Kit

3 h 30 min

GFAP is expressed exclusively in astrocytes in the central nervous system.The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

This kit recognizes Mouse GFAP in samples.No significant cross-reactivity or interference between and analogues was observed

0.78-50 pg/mL

Colorimetric method, ELISA, Sandwich

12 months

14580

Both intra-CV and inter-CV are < 10%.

80%-120%

0.47 pg/mL

Gfap

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GFAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 11 nm. The OD value is proportional to the concentration of Mouse GFAP. You can calculate the concentration of Mouse GFAP in the samples by comparing the OD of the samples to the standard curve.

Serum, plasma and other biological fluids

Sandwich-ELISA

P03995

This ELISA kit applies to the in vitro quantitative determination of Mouse GFAP concentrations in serum, plasma and other biological fluids.