Elabscience

Uncoated Chicken Cort(Corticosterone) ELISA Kit

Product Code:
 
E-UNEL-Ch0002
Product Group:
 
ELISA Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Target Species:
 
Chicken (Gallus)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Ice bag
Storage:
 
-20°C
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Assay Time:
24 h
Cross Reactivity:
This?kit?recognizes Chicken Cort in?samples.No?significant?cross-reactivity?or?interference?between Chicken Cort and analogues was observed
Detection Range:
3.125-200ng/mL
Detection Type:
Colorimetric method, ELISA, Competitive
Expiry date:
12 months
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Target Synonym:
CORT, Corticosterone
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken Cortisol are added to the micro ELISA plate wells. Chicken Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The concentration of Chicken Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Competitive-ELISA
Usage:
This ELISA kit applies to the in vitro quantitative determination of Chicken CCL6 concentrations in serum, plasma and other biological fluids.

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