Elabscience

Uncoated Human IL-13(Interleukin 13) ELISA Kit

Product Code:
 
E-UNEL-H0415
Product Group:
 
ELISA Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Ice bag
Storage:
 
-20°C
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This product comes from: China.
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Further Information

Assay Time:
24 h
Cross Reactivity:
This?kit?recognizes Human IL-13 in?samples.No?significant?cross-reactivity?or?interference?between Human IL-13 and analogues was observed
Detection Range:
15.625-1000 pg/mL
Detection Type:
Colorimetric method, ELISA, Sandwich
Expiry date:
12 months
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Target Synonym:
Interleukin-13, IL13, NC30, P600
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human IL-13. You can calculate the concentration of Human IL-13 in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
Cellular supernatant, Serum, plasma and other biological fluids
Type:
Sandwich-ELISA
UNIProt ID:
P35225
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human IL-13 concentrations in cellular supernatant, serum, plasma and other biological fluids

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