Procell system

AtT-20 Cell Line

Product Code:
CL-0028
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Applications:
Transfection host.
Background:
Tested and found negative for ectromelia virus(mousepox).
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
Ham's F-12K(PM150910)+15% HS+2.5% FBS+1% P/S(PB180120)
Gene expression:
Adrenocorticotropic Hormone(Acth)
Growth Properties:
Suspension with clustering
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Round (in strings)
Split Ratio:
5x10^5-1x10^6 cells/mL
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Tissue:
Pituitary gland

Documents

References

https://www.sciencedirect.com/science/article/pii/S0378111916309830