Elabscience

Calu-1 cell line

Product Code:
CL-0053
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Age Gender:
male, 47 years
Background:
This cell line is derived from an epidermoid carcinoma of the lung in a 47-year-old Caucasian male. The line can form epidermoid carcinomas in nude mice and tumours in steroid treated hamsters. Ultrastructure includes numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. This cell line contains the ras (K-ras) oncogene.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
McCoy's 5A(PM150710)+10% FBS+1% P/S(PB180120)
Gene expression:
Blood Type A; Rh+; HLA A10, A11, B15, Bw35
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Lung; Derived From Metastatic Site: Pleura
Tumorigenic:
Yes, in nude mice; forms epidermoid carcinomas. Yes, in steroid treated hamsters.

Documents