Procell system

CCRF-CEM [CCRF CEM] Cell Line

Product Code:
CL-0058
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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Further Information

Age Gender:
4 years juvenile
Antigen expression:
CD3; Homo sapiens, expressed; CD4; Homo sapiens, expressed; CD5; Homo sapiens, expressed; CD7; Homo sapiens, expressed
Applications:
Transfection host.
Background:
CCRF-CEM cells were derived from the peripheral blood buffy coat of a child(CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma. Do not centrifuge after thawing.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Doubling Time:
~24-30 hours
Effects:
Yes, in syrian hamsters.
Gene expression:
Cd3; Homo Sapiens, Expressed , Cd5; Homo Sapiens, Expressed , Cd7; Homo Sapiens, Expressed , Cd4; Homo Sapiens, Expressed
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
3x10^5-5x10^5 cells/mL
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
Tissue:
Peripheral blood
Tumorigenic:
Yes

Documents

References

  • https://www.nature.com/articles/s41467-025-67093-w
  • https://www.sciencedirect.com/science/article/pii/S0925400523006056
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947146/