Procell system

CFPAC-1 Cell Line

Product Code:
CL-0059
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
26 years
Antigen expression:
CA19-9 antigen, 12000 units/mL, epithelial keratins
Applications:
Transfection host.
Background:
The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene(cystic fibrosis transmembrane regulator, CFTR).CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca+ionophores with increase Cl- efflux.The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
IMDM(PM150510)+10% FBS+1% P/S(PB180120)
Doubling Time:
~30-45 hours
Effects:
Yes, in nude mice.
Gene expression:
Carcinoembryonic Antigen(Cea), 9Ng/Ml; Pancreatic Oncofetal Antigen(Poa), 28Ng/Ml; Adenocarcinoma Associated Antigen(Acaa), 5000Ng/Ml; Ca 19-9 Antigen, 12000 Units/Ml; Epithelial Keratins
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:3-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Pancreas; derived from metastatic site: liver
Tumorigenic:
Yes

Documents

References

  • https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202517184
  • https://www.embopress.org/doi/abs/10.15252/embj.2023114558
  • https://www.nature.com/articles/s41401-026-01809-6