Elabscience

EL4 Cell Line

Product Code:
CL-0078
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Antigen expression:
H-2b; Thy-1.2
Applications:
Transfection host.
Background:
The cells are resistant to 0. 1mM cortisol and sensitive to 20 ug/mL PHA. A subline(EL4. IL-2) that produces high levels of interleukin-2(IL-2, interleukin 2) is available. A subline(EL4. IL-2) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine(BUdR) is available. A subline(EL4. BU. 1. OUAr. 1. 1) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine and 1mM ouabain is available. Tested and found negative for ectromelia virus(mousepox).
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% HS+1% P/S(PB180120)
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Tissue:
Peripheral blood

Documents