Elabscience

H9c2(2-1) Cell Line

Product Code:
CL-0089
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Rat
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Embryo
Applications:
Transfection host.
Background:
Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation.Fusion occurs faster if the serum concentration in the medium is reduced to one percent.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Gene expression:
Myokinase; Creatine Phosphokinase; Myosin
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Myoblast
Receptor expression:
Acetylcholine, expressed
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Heart/myocardium

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