Procell system

HCT-8 [HRT-18] Cell Line

Product Code:
CL-0098
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
67 years adult
Background:
The HCT-8 line is identical to the HRT-18 cell line.The cells are positive for keratin by immunoperoxidase staining.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Effects:
Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
Gene expression:
Carcinoembryonic Antigen(Cea) 0.5Ng/10^6 Cells/10 Days; Alkaline Phosphatase. The Cells Are Positive For Keratin By Immunoperoxidase Staining.
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:3-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Large intestine; Colon
Tumorigenic:
Yes

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S2095927323007818
  • https://www.sciencedirect.com/science/article/pii/S221138352400008X
  • https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(22)00624-4